Orchis sancta L.'nin In Vitro Asimbiyotik Çimlendirilmesi, Fide ve yumrularının oluşturulması

Plant tissue culture techniques have recently become attractive tools for propagating rare and endangered species producing their economically important natural products. Here, we introduce a protocol an effective, fast reliable production route of plantlets tubers from salep orchid, Orchis sancta, native orchid growing wild in Turkey. In the course this study, efficacy basal media plant growth regulators on asymbiotic germination mature seed, seedling formation, tuberization glucomannan contents were assessed. Amongst all evaluated, Orchimax including activated charcoal gave best rate (62.46%). Supplementing medium with 1.0 mg/L zeatin resulted highest (81.18%). Lowest thidiazuron concentration (0.25 mg/L) was very effective shoot elongation while kinetin (0.5 promoted leaf formation. The aforesaid IBA favored root extension (31.16 mm) formation (4.5 roots per plantlet) at 2.0 IBA, respectively. Cytokinins, (2.0 particular, tuber accumulation. Plantlets successfully adapted to soil step by step.